The painting, Portrait of Eleanor of Toledo and Her Son Giovanni, was painted c. 1545 by Agnolo di Cosimo, known as Bronzino. The painting is of Eleonora di Toledo, the Duchess of Florence, and her son Giovanni. This portrait uses the position of the two and their clothing to exemplify her power, fertility, and the legacy of the Medici family. It is housed in the Uffizi Gallery of Florence, Italy and is considered one of the preeminent examples of Mannerist portraiture. Bronzino, 1544 — painted at 41. Panel and oil paint. 115 × 96 cm. In the Uffizi Gallery. Mannerism. Wiki link